Separation of helper and suppressor T lymphocytes on a ficoll velocity sedimentation gradient.
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چکیده
منابع مشابه
Separation of helper and suppressor T lymphocytes on a ficoll velocity sedimentation gradient
A 5-20% Ficoll velocity sedimentation gradient has been successfully applied to separate concanavalin A (Con A)-induced helper; and suppressor T cells. When titrated into a constant number of fresh normal spleen cells responding to sheep erythrocytes, cells from the top pool show stimulatory effects while those from the bottom pool show inhibitory activity. Both activities are found to be Con A...
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The existence of subpopulations of T cells has been recognized in a number of experimental models. Raft and Cantor (1) first subdivided T cells into T1 and T2 according to an antigen-driven maturation scheme. T1 cells which were immature, short-lived, and noncirculating upon encounter with antigens developed into mature T~ cells which were long-lived, circulating, and had a lower density of Thy...
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Using a Ficoll velocity sedimentation gradient, we have been able to fractionate concanavalin A (Con A)-induced helper and suppressor cells into separate pools. Cells activated by Con A to mediate helper activity are Ly1+, do not require DNA synthesis for induction, and remain as small cells after activation. Suppressor cells are Ly23+, are found in the blast cell fraction and their induction i...
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The effects of dexamethasone (Dex) on the functions of antigen-primed helper and suppressor T cells were studied in humoral immune responses in vitro. In doses equivalent to elevated physiologic concentrations, the suppressor T cell activity was abolished. In contrast, the helper T cell function was resistant to even pharmacologic concentrations of Dex. The apparent steroid resistance of the he...
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Data presented here show that locidentify in the I-region of the H-2 gene complex are selectively expressed in different functional T-cell subpopulations. These loci are closely linked (or possibly identical) to loci that control immune responses. They control surface determinants which identify helper and suppressor T lymphocytes. Determinants described here on allotype suppressor T cells (Ts)...
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ژورنال
عنوان ژورنال: Journal of Experimental Medicine
سال: 1976
ISSN: 0022-1007,1540-9538
DOI: 10.1084/jem.143.5.1199